###06A_06B_06C_06D_06E### wciP 06A, 06B #snps discriminating 06A and 06B wciP 06C, 06D #snps discriminating 06C and 06D ###07A_07F_40### wcwD 07F: normal, 07A: pseudo#insertionA587 #frameshift mutation using this wcwD-spc07F to discriminating 07F and 07A wcwK 07B, 07C, '40' #snps ###09A_09L_09N_09V### wcjE 09V: normal, 09A: pseudo#deletionG722 #frameshift mutation using this wcjE-SPC09V to discriminating 09V and 09A wcjA 09L, 09N #snps not use #wcjB 09L, 09N #snps not use ###10A_10B### wcrC 10A, 10B #wrcC was different in all four serotype (10A,10B,10C and 10F), but this gene was only used for discriminating 10A and 10B ###11A_11B_11D_11F### wcrL 11A, 11D, 11F #snps using this gene to discriminate 11A from 11D and 11F ###12B_12F### wciI-SPC12F 12B, 12F #allele using wciI-12F to discriminating 12F wcxF-SPC46 12A, 46 #snps wcxF-SPC46 was used to discriminating 46 and 12A ###15A_15B_15C_15F### wciZ 15B: normal, 15C: pseudo#repeatTA413 #frameshift mutation used to discriminating 15B and 15C ###18A_18B_18C_18F### wcxM 18F: 1, 18A: 0, 18B: 0, 18C: 0 #presence used to obtain 18F wciX 18C: normal, 18B: pseudo#stopcodon #frameshift mutation used to discriminating 18B and 18C ###19A_19AF### wzy 19A, 19F, 19C, 19B #allele used to discriminate 19A and 19F ###22A_22F### wcwA 22A: 1, 22F: 0 #absence this gene was used to obtain 22F which does not contain wcwA. (Note: reference 22F have this gene) ###23A_23B_23F### wchA 23A, 23B|23F #allele wzy 23A, 23B, 23F #allele ###25A_25F_38### wcyV 25A: 0, 25F: 0, 38: 1 #presence used to obtain 38 ###28A_28F### wciU 28A, 28F #snps ###33A_33F_33B_33D### wciB 33F: 1, 33B: 0 #presence used to obtain 33F wcjE 33A: 1, 33F: 0 #absence pseudo#inserionT433 in 33F that causing frameshift mutation. /** When predict serotype is 33F, wcjE gene is the key choice for determinate serotype **/ wciN-SPC33B 33B, 33D #presence wciN-SPC33B used to discriminating 33B and 33D ###35A_35C_42### wcrX 35C: normal, '42': normal, 35A: pseudo#insertionA248 #frameshift mutation wciG 35D: 0, 35B: 1 #absence used to obtain 35D ###41A_41F### wcrX 41F: normal, 41A: pseudo#deletionG23 #frameshift mutation