SUBID     	BioProject	BioSample	Organism
--------------------------------------------------------
SUB13578731	PRJNA989674	SAMN36186457	Serratia marcescens

[] We ran your sequences through our Contamination Screen. The screen found 
sequences that need to be trimmed and/or excluded. More details about the 
contamination screening process are available at https://github.com/ncbi/fcs

We were able to remove sequences marked to exclude and/or trimmed contamination 
from the ends of sequence (see the FixedForeignContaminations.txt file). If the 
contamination is on either side of a run of N's, the N's were also removed.
We have uploaded the modified fasta file ForeignContaminationModified.zip to the 
submission portal.

However, we could not remove the contamination in the RemainingContamination.txt file.  
We cannot remove contamination that is in the middle of a sequence. The sequence may need
to be split at the contamination and the sequence on either side of the contamination submitted 
as a separate sequence. In addition, we do not automatically remove mitochondrial 
sequences in eukaryotic submissions. 

Please adjust these remaining sequences appropriately and then resubmit.  After you 
remove the contamination, trim any Ns at the ends of the sequence and remove any sequences 
that are shorter than 200 nt and not part of a multi-component scaffold.

Note that mismatches between the name of the adaptor/primer identified in the screen 
and the sequencing technology used to generate the sequencing data should not be used 
to discount the validity of the screen results as the adaptors/primers of many 
different sequencing platforms share sequence similarity.


Adaptor:
[] Some of the sequences hit primers or adaptors used in Illumina, 
454, or other sequencing strategies or platforms.  Adaptor at the 
end of a sequence should be removed. However, if adaptors are 
present within sequences then you should strongly consider 
splitting the sequences at the adaptor because the adaptor sequence 
could have been the region of overlap, causing a misassembly.


The contamination that we could not automatically remove is listed below.
You will need to adjust these sequences and resubmit:

Skipped 111 same as before; no new sequences to screen.
Note: 11 sequences with runs of Ns 10 bp or longer were split before comparing.
1 sequence to trim

Trim:
Sequence name, length, span(s), apparent source
SM47_1_5	377	204..273	adaptor:NGB00752.1-not_cleaned


